![]() Screens SYBR® Green primers for thermodynamic properties and secondary structures.Īvoids homologies with all other primers to reduce primer dimers in a multiplex reaction.ĭesigns optimal TaqMan® probes free of dimers, repeats and runs to ensure signal fidelity.ĭesigns wild and mutant TaqMan® probes for SNP identification for SNP genotyping assays Presents the design results in a ranked order. Pre-designed primer sets can be evaluated and a compatible TaqMan® probe be designed. Similarly, a pre-designed TaqMan® probe can be evaluated and compatible primers be designed. Beacon Designer™ can design LNA™ probes.You can even import the primers designed in the SYBR® Green primer design mode and design a compatible probe.ĭisplays a graphical view of TaqMan® probe secondary structures. LNA™ substituted TaqMan® probes are shorter probes and have LNA™ bases that impart higher stability. You can specify the number of LNA™ bases that you wish to have in the probe. ![]() ![]() You can also specify their placement and the LNA free region on each end of the probe to design LNA™ spiked TaqMan® probes. Read about how TaqMan® probes work! Multiplex Real Time PCR Assay Designīeacon Designer™ uses innovative proprietary algorithms to design optimal primer-probe sets for single tube multiplex assays. Multiplex assays are supported in the TaqMan® design mode. ![]()
0 Comments
Leave a Reply. |